10x product on white background

GemCode platform from 10X Genomics

DNA library preparation reagents from Kapa Biosystems Inc, Wilmington, Mass, have been incorporated into the GemCode platform from 10X Genomics, Pleasanton, Calif, making possible a more streamlined workflow for generating high-quality sequencing data.

The GemCode platform is a unique molecular barcoding and analysis platform that consists of instrumentation, reagent, and software to generate a new data type: linked reads. Using DNA inputs as low as 1ng, linked reads deliver long-range information, including haplotype phasing, structural variation, and other critical genomic information.

Kapa Biosystems uses a proprietary directed-evolution technology platform to engineer enzymes for the specific performance and functional requirements of next-generation sequencing sample preparation. The GemCode platform incorporates both the Kapa Hyper prep kit and Kapa HiFi DNA polymerase in the library construction process to make possible high-efficiency adapter ligation and low-bias library amplification.

One of the most powerful attributes of the GemCode platform is that it integrates with the large installed base of existing short-read sequencers to generate genomic data that preserve long-range information, says Kevin Ness, chief technology officer of 10X Genomics. It was a straightforward decision to include Kapa Biosystems in the GemCode platform based on the positive performance experienced by existing sequencing customers along with our own internal evaluation.

John Foskett, Kapa Biosystems.

John Foskett, Kapa Biosystems.

Kapa is excited to be working with 10X Genomics and recognizes the value of using linked reads for determining structural variation and haplotype information, which is not possible with current short-read sequencers, says John Foskett, chief technology officer of Kapa Biosystems. Our high-efficiency library preparation reagents are designed to meet the performance requirements of demanding new applications. These products are an ideal fit for the GemCode platform.

For more information, visit 10X Genomics and Kapa Biosystems.