Thermo Fisher Scientific Inc. announced the launch of ABsolute^™ Fast QPCR Master Mixes for the rapid and reliable probe-based detection of DNA and cDNA targets. ABsolute Fast QPCR Master Mixes have been specially developed for Fast QPCR protocols to deliver high performance QPCR results, on all major QPCR platforms, in a fraction of the time previously required^. 

By minimising QPCR protocol time¹, ABsolute Fast QPCR Master Mixes help to maximise throughput while delivering unbeatable² reproducibility, sensitivity and specificity. The mixes contain Thermo-Fast^™ DNA Polymerase, which can be activated in just 5 minutes at 95ºC (compared to 15 minutes required for traditional QPCR master mixes). Further time savings are achieved by optimising the temperature of the combined annealing and extension step, and significantly reducing dwell times at each temperature, allowing the fast QPCR protocol to be completed in less than one hour 

ABsolute Fast QPCR Master Mixes improve the performance of fast QPCR reactions when compared to other leading fast QPCR mixes, demonstrating increased sensitivity (lower Ct values), superior reproducibility of replicates and a more linear dynamic range^. 

ABsolute Fast QPCR Master Mixes are compatible with any sequence-specific probe. In addition to accelerating QPCR analysis of DNA, the master mixes can also be used for QRT-PCR analysis of RNA targets and are ideal for use downstream of the Thermo Scientific Verso™ cDNA synthesis kit or any other reverse transcriptase kit.