This L-Type UIBC reagent is liquid, ready-to-use and highly stable. The assay features excellent sensitivity and precision, with no significant interference by ascorbic acid, bilirubin, hemoglobin or intrafat. The within-run precision is 0.65 percent CV and 0.85 percent CV at mean concentrations of 183.6mg/dL and 102.3mg/dL, respectively. The Total precision is 5.28 percent CV and 2.95 percent CV at mean concentrations of 115.8mg/dL and 187.6mg/dL, respectively. The minimum detectable limit is 2mg/dL with a linearity limit of 600mg/dL.
     The L-Type UIBC assay is based on a method that utilizes bathophenanthroline (BPT) as a chromogen. When a sample is mixed with Buffer containing a known excess amount of iron, unsaturated transferrin in serum quantitatively associates with iron in the buffer and is converted to a saturated state. The portion of iron that remains unassociated is assayed through color development. UIBC concentrations are measured spectrophotometrically at 545nm. This assay can be automated on your existing clinical chemistry instrumentation.
Wako
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Keywords: anemia