Millipore now offers two high-sensitivity chemiluminescent substrates for western blotting applications: the Immobilon western HRP and the AP chemiluminescent. Both substrates provide high signal response at midfemtogram levels.
     Primarily used in western and dot/slot/spot blotting applications, each versatile substrate has a high signal intensity to facilitate the detection of both high- and low-abundance proteins. The western HRP and AP chemiluminescent substrates use two to five less primary and secondary antibodies than traditional, low-sensitivity substrates, resulting in decreased antibody consumption.
     Available in ready-to-use formats, the two substrates are compatible with polyvinylidene fluoride (PVDF), nitrocellulose membranes, and they commonly used buffers and blocking reagents.

Millipore Corp
(978) 715-4321; www.millipore.com/immobilon