Polypus-transfection has released a DNA reagent that is efficient in a wide variety of eukaryotic cells. A polymeric transfection reagent, jetPEI™ has proven successful in delivering DNA to more than 200 adherent and non-adherent cell lines and is consistently ranked among the top two best DNA carriers. It is appropriate for routine transfection and particularly suitable for fragile, slow-dividing cells, such as primary cells. jetPEI™ is a polyethylenimine that behaves as a proton sponge by absorbing the acidity of the DNA-containing endosomes. As a result, DNA is protected and vacuole swelling causes rapid release of the jetPEI™/DNA complexes into the cytosol. And unlike lipidic transfection reagents, jetPEI™ is a water-soluble polymer that enables consistent complex formation with DNA. Complexes remain stable for as long as 2 hours, helping minimize intra- and inter-assay variability and maximizing reproducibility. The jetPEI™ transfection protocol is simple and straightforward and does not require any medium change or washes. Optimized protocols for HTS and protein production are also available. Polypus-transfection also offers labeled jetPEI™ reagents for investigating delivery efficiency, intracellular trafficking, and sorting complex-containing cells.

Polyplus-transfection (760) 481-4918;
www.polypus-transfection.com