The Clontech™ Antibody Array Nanoscale Buffer Kit is designed for those who have limited amounts of sample and want to assay hundreds of cellular proteins in a single experiment. Ideal for use with the Clontech™ Ab Microarray 500, the kit offers a gentle, nondenaturing method for preparing total protein extract from extremely limited samples, including fine needle aspirate, serum, tissue, and as few as 1,000 cells. The Nanoscale Buffer Kit provides a novel method of extracting and fluorescently labeling proteins from limited samples. The protocol saves sample and can be completed in just 1 day—from sample preparation to data acquisition. Specialized training is not required, and the slides are compatible with commercially available fluorescence scanners. The extraction/labeling buffer is optimized for labeling with N-hydroxysuccinimide (NHS)-ester dyes (including Cy3 and Cy5 dyes). The extraction/ labeling buffer, incubation, and wash buffers are formulated to minimize nonspecific background binding and yield a high signal-to-noise ratio.

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